Step 1: Enter Sequence Input

You can input DNA in raw text, FASTA, GenBank ID formats.

Fig 1: User enters a sequence in text format and clicks “Analyze”.

Step 2: Review Sequence Statistics

Once analysis begins, the first section of results displays useful sequence statistics:

• Base counts (A, T, G, C)
• AT/GC content
• Molecular weight
• Melting temperature

Fig 2: Sequence statistics output with key metrics.

Step 3: View Cut Site Visual Map

This interactive map shows where each restriction enzyme cuts the top and bottom strands. Hover for tooltips and scroll to zoom or pan.

Fig 3: Visual map showing enzyme cut positions on the sequence.

Step 4: Interpret Cut Site Table

The table provides an overview of:

• Enzyme name
• Recognition sequence
• Cut site length
• Frequency of cuts
• Top and bottom strand cut positions

Fig 4: Tabular view of all identified cut sites.

Troubleshooting

• Ensure correct formatting for FASTA or ID-based inputs
• Use smaller sequence portions for better visual clarity

Still Need Help?

Visit the Contact Us page or email us. Feel free to ask.